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Human peripheral blood mononuclear cells PBMC from healthy donors, hyperthyroid GD patients and euthyroid GD patients were isolated from heparinized peripheral blood by density gradient centrifugation on Ficoll-Hypaque (Sigma Aldrich)

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Furthermore, the plasma level of resistin in PCOS patients is higher than in healthy people, contributing to the emergence of insulin resistance and hyperinsulinemia in these patients