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Immunolabeling assay for Cntfr and lumenal domain of Syt1 To label Cntfr, 15 DIV primary hippocampal neurons were incubated with rabbit anti-CNTFRα (extracellular) antibodies (1:50 dilution, Alomone ACR-051, RRID: AB-2340925) in conditioned media at 37 °C for 40 min