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This results in a condition not only with elevated homocysteine, but also more severe symptoms affecting the nervous system, connective tissue, muscles, and cardiovascular system

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Reactions consisted of 5 μl of uronate dehydrogenase (1 μm final), 5 μl of enzyme (30 nm final), 10 μl of NAD + (2 mm final), and 30 μl of E1-3G or E2-17G (500 μm final), all diluted in assay buffer (50 mm HEPES, 50 mm NaCl, various pH) (Fig