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Oxaloacetate functions as an intermediate in several metabolic pathways, including gluconeogenesis, the glyoxylate cycle, amino acid synthesis and fatty acid synthesis [103]

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In detail the protocol starts with a 10-min incubation, in room temperature, in the dark, of 40 μL PBS (Phosphate Buffer Saline, pH 6.8), 20 μL of extract (200 μg/mL) and 20 μL of tyrosinase enzyme (Sigma Aldrich) at final concentration of 92 U/mL, loaded in a 96 well plate