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Non-reducing SDS-PAGE showed long smears for the oligomeric species of E1E2 and E2E1, indicating the presence of heterogeneous disulfide formation 31 , while E2E1-I53-50A showed one distinct band larger than 250 kDa, suggesting that E2E1-I53-50A forms a disulfide-bonded trimer (Fig

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tuberculosis invasion, macrophages deploy a repertoire of defense strategies, including phagosome-lysosome fusion to degrade pathogens, recruitment of hydrolytic lysosomal enzymes, production of reactive oxygen species (ROS) and reactive nitrogen species (RNS), antigen presentation, upregulation and mobilization of major histocompatibility complex class II (MHC class II) molecules, autophagy, and induction of apoptosis ( M