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Validating these findings, deletion of the Mitochondrial Pyruvate Carrier—which is known to decrease TCA cycle activity and protect the liver from steatohepatitis—also diminished NADPH, elevated glutathione oxidation, and alleviated ER stress

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In each slice, the region within the mCherry-LMNB1 boundary in the red channel defined the nuclear region, and was automatically selected in each slice by ImageJ script