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2.4 Plasmid construction and plant transformation To construct recombinant plasmids, the target genes were cloned into the Pro35S :pRI101-AN, Pro35S :pCAMBIA1302, Pro35S :pTRV2, Pro35S :pET22b, PSYCE-35S, and PSYNE-35S plasmids by seamless cloning after plasmids were linearised using restriction enzymes (New England Biolabs, Beijing, China)
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The advanced bi