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Then the samples were mounted in a Monolith NT.115 instrument with blue/red filters (NanoTemper Technologies, Germany) for binding measurements, which were performed at 25 °C using 20% blue LED excitation power (blue filter, excitation 460–480 nm, emission 515–530 nm) and 60% IR-laser power with off/on times of 3 s and 20 s

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