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The FHC cells were cultured in a 1:1 mixture of Ham's F12 and Dulbecco's modified Eagle's medium (Gibco Life Technologies) supplemented with 25 nM HEPES (Gibco Life Technologies), 10 ng/ml cholera toxin (EMD Millipore, Billerica, MA, USA), 5 μg/ml insulin (Sigma-Aldrich, St