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After binding, the cartridges were washed once with 250 µL of DUB buffer containing 2% SDS and twice with 250 µL of 50 mM ammonium bicarbonate (AmBic) at 10 µL/min

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GHK-Cu has been applied to osteoblast precursor cell cultures (e.g., MC3T3-E1 cells) to investigate its role in osteogenesis