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Mammalian PUS family members exhibit a fine subcellular division of labor: In the cytoplasmic system, PUS10 has been shown to specifically catalyze Ψ-modification at tRNA positions 54/55 (124,125), whereas PUS1 regulates transcript stability by recognizing mRNA stem-loop structures (119,126)

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While most of these are still in the preclinical ‘tool compound’ stage or have been limited by toxicity, allosteric inhibitors of GLS have shown promise in preclinical models of cancer, and one highly potent compound in this class, CB-839, has moved on to clinical trials