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Lysates were resolved by sodium dodecyl sulfate-polyacrylamide gel electrophoresis using 4–12% gradient NuPAGE gel in MOPS buffer (Life Technologies) and transferred to nitrocellulose membranes for Western blotting

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Search Strategy A comprehensive search of PubMed/MEDLINE and the Cochrane Central Register of Controlled Trials (CENTRAL) was performed from database inception through the final search date