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Distal colon content (200 mg) from two mice for one experiment was homogenized in PBS (1 mL) and centrifuged at 9000 g , the supernatant was collected, and then the low-molecular-weight fraction of the supernatant was fractionated using Amicon Ultra (Ultracel 3 K, Merck, Darmstadt, Germany) and the high-molecular-weight fraction was fractionated using a PD SpinTrap G-25 column (GE Healthcare, Buckinghamshire, UK)

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Size:50 ml Vial (Multi Dose)

PDK1: pyruvate dehydrogenase kinase 1