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Then, the sections were washed with 0.1 M PB, endothelial cells were counterstained with lectin, and nuclei were counterstained with 4′,6-diamidino-2-phenylindole (DAPI, Invitrogen), according to the manufacturer’s protocols

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Qualitatively and quantitatively the same results were obtained when the TYO intermediate was not isolated by extraction and a second equivalent of a different thiol was just added in the initial reaction medium (Fig