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Barcode-based demultiplexing was performed using IDEMP software with default parameters ( Obtained reads were assembled, quality controlled, and clustered using Usearch8.1 software package ( Briefly, reads were merged using -fastq_mergepairs –with fastq_maxdiffs 30 and quality filtering was done with fastq_filter (-fastq_maxee 1), minimum read length 200 bp

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Fresh blood remains the most suitable sample type for analyzing physiological levels of phosphorylated metabolites