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The authors could confirm and extend the observations from the group of Lutz in so far as the clustering agent was only effective upon subsequent crosslinking of aggregated band 3 protein and only then induced binding of autologous IgG, deposition of C3c and phagocytosis (Turrini et al., 1991)

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Storage & Handling (Laboratory Guidance) Store lyophilised material in a cool, dry environment away from light Follow batch-specific documentation for stability guidance After reconstitution with an appropriate laboratory solvent, store under refrigerated conditions Avoid repeated freeze–thaw cycles to maintain sample integrity Always adhere to institutional SOPs and product documentation