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The following primers to amplify the fragment were designed using the homologous recombination method: GRFT-SmaI(F): 5′-GtctcgaggaccggtcccgggATGAAGGCTTTCACTCTTGCCT-3′ and GRFT-PmlI(R): 5′-ctggtcaccaattcacacgtgATACTGCTCGTAATAGATATCAAGGGA-3′

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In this manner, the “GSH cycle” takes place (Figure 2B), which includes the synthesis, cellular distribution, and metabolism of the molecule [21]