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MS 2 scans were operated with a higher-energy collisional dissociation of 35% at 50,000 mass resolution, 1 × 10 5 automatic gain control target and 86-ms maximum injection time, with an isolation window of 0.7 m / z

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In Southern blotting, genomic DNA (typically fragmented and separated on an electrophoretic gel) is hybridized to a probe specific for the target region