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The protein-bound resin was extensively washed with 100 mL of buffer consisting of 20 mM Tris-Cl (pH 8.0), 500 mM NaCl, 1 mM DNQX, and 0.02% DDM at room temperature, and then slowly washed with 200 mL of buffer consisting of 20 mM Tris-Cl (pH 8.0), 300 mM NaCl, and 0.02% DDM at room temperature at a rate of approximately 0.3 ml/min

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