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Later on samples were oven-dried (48 h) to get dry weight (W 3 ), and relative water content (RWC) was calculated by using the following equation: Quantification of photosynthetic pigments Fresh leaves samples (0.5 g) were extracted in 80% C 3 H 6 O (5 mL)

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On the other hand, a significant loss of total GLs content (−59%) in tatsoi leaves boiled for 5 min, when compared with raw samples, was observed (Yang et al., 2019)