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Overall, these findings indicate that reductions in GPx3 abundance trends with disease severity with stage 1 having the highest mean levels of GPx3 (1.99 µg/ml ± 0.73 µg/ml) and the highest K1/U1 ratio (0.86), followed by the stage 2 with a mean of 1.2 µg/ml ± 0.54 µg/ml and average K2/U2 ratio of 0.67, with the most severe form of konzo, stage 3, displaying the largest reduction in average protein abundance (mean: 1.15 µg/ml ± 0.58 µg/ml) and the lowest K3/U3 ratio of 0.59 (Supplementary Data 1)