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GLUTATHIONE AS AN ANTIOXIDANT Oxygen radical stress occurs in all aerobic organisms as a result of aerobic metabolism, with intermediates such as superoxide and hydrogen peroxide that lead to further production of oxygen radicals, which can cause lipid peroxidation and disrupt metabolic processes

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After treating cells with single drugs for the appropriate duration (0-72h), they were incubated in serum-free medium for 15 min in 0.5 μM 5-CMF, then washed by centrifugation, and stained with 1 μM PI to further exclude any dead cells from the analysis