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This increase of insulin receptors and GLUT4 proteins subsequently led to improved glucose tolerance, increased insulin sensitivity, and noticeably reduced markers of oxidative stress, as observed by low malondialdehyde (MDA) levels (biomarker for oxidative stress) ( In females with PCOS, magnesium supplementation is associated with decreased oxidative stress and inflammation when combined with zinc, calcium, and vitamin D, owing to decreased levels of hs-CRP and MDA in addition to decreased levels of insulin resistance indices ( Calcium: insulin signaling, ovarian function, and metabolic homeostasis A vital intracellular second messenger, calcium is involved in the synthesis of steroid hormones, follicular maturation, and insulin secretion
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To confirm the importance of RFK and FLAD1 in FSP1 regulation, we generated RFK -KO and FLAD1 -KO cells (RFK KO and FLAD1 KO cells) in the FSP1 GFP-P2A-BFP cell line
