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To establish a linear curve for the detection of MNA peak, a 10-0.3125 uM/100 μL half-fold serially diluted samples of MNA were prepared in reaction buffer containing 1 mM Tris [pH 8.6], 1 mM DTT, 10% trichloroacetic acid, 4% methanol, and water

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As a permanently charged quaternary cation it is water-soluble and does not require organic cosolvent for aqueous work, though DMSO stocks are common in cell-based screens