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Briefly, mycelial fragments or conidial suspensions were incubated with 10 μM DCFH-DA at 28 °C for 30 min in the dark, washed three times with phosphate-buffered saline (PBS) 64 , and immediately observed using fluorescence microscopy (Axio Imager M2, Zeiss) with excitation/emission wavelengths of 488/525 nm

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Use the storage temperature and handling instructions printed on the exact BAC water product