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Slides were further washed twice for 5 min each with buffer B (100 mM Tris-HCl, pH 7.0

diet

Size:1000 mcg/ml - 10 ml (Pack of 3)

Next, 100 μM TAMRA-PEG 3 -N 3 , 200 μM Cu(II)SO 4 , 1 mM THPTA and 5 mM freshly dissolved sodium-ascorbate were added to the mixture and it was further incubated at ambient temperature, under constant agitation (300 rpm) for overnight