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Generation and quantification of protein persulfides and polysulfides in cell lysates HEK 293 cell lysate (200 µg) protein/sample in 50 mM HEPES buffer (pH 7.4) was treated with Na 2 S (0–40 µM) or Na 2 S 2 (0–600 µM) for 7 min in sealed Eppendorf tubes with minimal head space at RT

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