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Supplemental double staining was used for the distinction between viable, apoptotic and necrotic cells with YO-PRO-1 / PI (Molecular Probes) in parallel with annexin-V/PI staining done with annexin-V-FITC when needed (Immunotech, Beckman-Coulter)

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The transcriptome data under the copper ion stress of GXDK6 has been uploaded to the SRA database of the NCBI database, the accession number is PRJNA752222, visit the website to obtain the data details