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Use either of the following options to remove residual ethanol from the eluate: re-purify the sample using a QIAquick-, or MinElute column, or QIAEX II resin incubate the eluate at 56°C for 10 min to evaporate the ethanol dry down the sample in a vacuum centrifuge, and resuspend the pellet in a small volume of sterile water FAQ-205 Buffer PB contains a high concentration of guanidine hydrochloride and isopropanol