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To better understand the pathogenic role of an altered kidney macrophage LIP versus ferritin-stored iron in kidney fibrosis, we next examined the effect of FtH suppression in macrophages by inducing CKD in myeloid specific Fth1 -knockout mice ( Fth1 LysM+/– ) (Figure 2 and Supplemental Figures 4–6)

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Following overnight incubation at 4 °C with primary antibody (1:500 in blocking buffer) cells were washed with the same buffer 3 times for 15 min each