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The inner phase of the scaffold was a polycaprolactone triol malate (PPCLM) matrix oriented in concentric sheets and seeded with chondrocytes to recapitulate the inner layer of the AF, which is rich in type II collagen and proteoglycan

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For immunohistochemistry, sections were developed using DAB (3,3’-diaminobenzidine) for color visualization, followed by counterstaining with hematoxylin

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