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Cells were washed four times for 2 min in PBS/1% BSA

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EGFR signaling: Compressive forces from the airway smooth muscle cell contraction during bronchoconstriction and pro-inflammatory cytokine TNF-α from eosinophils and neutrophils upregulate the expression of EGFR and its ligand heparin-bound-EGF (HB-EGF) in the injured airway epithelial cells (83), leading to activation of EGFR signaling, as shown in Figure 7