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Human monocytic leukemia THP-1 cells were maintained in RPMI 1640 media supplemented with 2 mM L-glutamine (Gibco, Thermo Fisher Scientific), 10% FBS, 5% penicillinโstreptomycin (Gibco, Thermo Fisher Scientific), and amphotericin B (Gibco, Thermo Fisher Scientific)
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It appeared to be hemoglobin, and this marker was subjected to additional analysis, considering a possible nonlinearity of this dependency
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