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The kit’s microplate wells are pre-coated with a monoclonal capture antibody targeting the N-terminal domain of rat GSH-PX, while a biotinylated polyclonal detection antibody binds the C-terminal domain—this dual-epitope recognition ensures exclusive reactivity with rat GSH-PX, eliminating cross-reactivity with human/mouse GSH-PX or rat glutathione S-transferase (GST)

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