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Perfusate samples were collected every minute and stored at −80 °C

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Media crowded with macromolecules such as high molecular weight dextran sulphate (0.01% w/v) was reported to increase collagen synthesis by embryonic pulmonary fibroblasts 20–30 fold, compared to normal media 31 and soluble procollagen was completely processed into insoluble collagen by dermal fibroblasts 39 , although the collagen was deposited as aggregates and not fibrils 30