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Tissues were incubated in enzyme mix (Miltenyi Biotec, spleen dissociation kit, 130-095-926) at 37 °C for 15–30 min with agitation every 5 min to promote enzymatic digestion of extracellular matrix proteins

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Another relatively new theory, that is rapidly gaining traction, is cellular toxicity caused by intracellular protein misfolding and aggregation 2,5,6,7