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To address this question, we extracted DNA from three kratom samples with varying speciofoline content (K51, K52 and K55 in Table 1), and used the resulting data for DNA barcoding via BLAST search of rbcL + matK (core plant barcoding markers) and performed maximum likelihood and Bayesian phylogenetic analysis, of the combined matK + trnH-psbA and the separate nrDNA ITS regions