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Interferon-γ (IFN-γ) was an important cytokine, and activation of IFN-γ could down-regulate the downstream JAK-STAT3 pathway, resulting in STAT3 phosphorylation on tyrosine 705, initiating the PD-1/PD-L1 axis and helping tumor cells escape immune surveillance and immune killing [97]

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The immune cells in the portal area of the untreated mice had increased expression of cleaved caspase-3 an executioner enzyme in the process of apoptosis 23,24,25