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Wellness · 20 July 2025 · 7 min read Needle Size for a B12 Injection: A Simple Guide By Alaiyka Parvez Owner, CoLaz Aesthetics Clinic The short version • For an intramuscular B12 injection, the usual choice is a 22 to 25 gauge needle, 1 to 1.5 inches long, given at 90 degrees into the deltoid