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32 , 33 Beyond these compositional proteins, the tight junction is functionally and structurally linked to the subcortical terminal web of actin microfilaments and the perijunctional actomyosin ring.34, 35, 36, 37, 38, 39 Solutes and water cross the tight junction by two distinct pathways that can be distinguished on the basis of their size-selectivity, charge-selectivity, and capacity (Figure 1 A )

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