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Activity of enzyme was determined in 100 mM potassium phosphate buffer (pH 6.5) with 1 mM l-chloro-2,4-dinitrobenzene (CDNB) and 4 mM GSH as substrates by following the change in absorbance at 340 nm (ε 9.6 mM −1

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In summary, KIT mutations (excluding mutations in exon 8), multiple aneuploidy, polyploidy, and accumulation of gene mutations and chromosomal and subchromosomal copy number variants are associated with aggressive, higher-grade MCTs and a negative prognosis