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2.2 Vitrification and warming The domestic cat ovarian cortex vitrification method was based on published article ( 2.3 Culture Fragments were cultured in MEM medium supplemented with the following components: 50 μM ascorbic acid, 0.3% (w/v) polyvinyl alcohol, 1% (v/v) insulin-transferrin-selenium (ITS) mixture, 10 μg/mL follicle stimulating hormone (FSH), and 1% (v/v) penicillin-streptomycin solution