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l-tryptophan is an essential AA, vital for biosynthesis of serotonin (5-HT), melatonin and co-factor NAD + through its downstream metabolic pathways 425

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So, we used the ImageStream system on selected double-stained cells to analyze the colocalization of LysoTracker Red and LC3B green immunostaining, which would reflect the formation of autolysosomes (Fig 6A)