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Preclinical: In a comparative study assessing the protective benefits of IN-EPO versus invasive intracerebroventricular (ICV) administration in an Aβ 25–35 non-transgenic mouse model of AD, ICV-EPO at a dose of 125–500 µg/kg, IN-EPO at a much lower dose of 62–250 µg/kg, and low sialic form termed ‘Neuro-EPO’ (also intranasal) significantly prevented Aβ-induced learning deficits, hippocampal neurodegeneration, and reduced levels of neuroinflammatory markers tumor necrosis factor (TNF) and interleukin-1β (IL-1β) [30]