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Although the initial objective of these studies was to understand the mechanism of this pump in plants, recent investigations in ours and other laboratories have demonstrated these pumps in organisms as disparate as thermophilic Archaea and parasitic protists

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Membranes were blocked with 5% milk in TBST and then incubated with primary antibodies overnight at 4 °C (all antibodies were 1:1000 in 5% milk in TBST, while GAPDH primary antibody is diluted 1:5000 in 5% milk in TBST)