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Furthermore, in vitro studies have demonstrated that high-glucose conditions dose-dependently suppress PPARα activity in astrocytes, increasing their susceptibility to ferroptosis, and gemfibrozil, a PPARα agonist, inhibits ferroptosis in db/db mice and astrocytes [160]

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The membranes were blocked in PBST (PBS supplemented with 0.1% Tween 20) containing 5% skim milk at room temperature (RT) for 1 hour