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Our study provides a valuable resource of protein targets of itaconate in macrophages and establishes a negative-feedback link between glycolysis and itaconate, elucidating new functional insights for this anti-inflammatory metabolite

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Epithelial cells from Lgr5 DTR-EGFP/+ organoids were gated as EpCAM⁺CD45 − CD31 − Ter119 − 7AAD − , and Lgr5-EGFP fluorescence intensity was used to classify cells into GFPhigh and GFPneg populations, where the GFPneg gate was defined based on the background fluorescence intensity of WT control organoids lacking EGFP expression