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The amide column re-equilibration gradient was as follows: 0-1 min held at 30% B at 0.4 mL per min, 1-3 min from 30% B to 65% B and 0.4 mL per min to 0.8 mL per min, 3-15 min held at 65% B and 0.8 mL per min, 15–15.5 min from 65% B to 100% B at 0.8 mL per min, 15.5–17 min held at 100% B at 0.8 mL per min, 17–17.5 min from 100% B to 95% B and 0.8 mL per min to 1.2 mL per min, 17.5-19.4 min held at 95% B and 1.2 mL per min, 19.4-19.8 min held at 95% B and decreased from 1.2 mL per min to 0.4 mL per min, and 19.8-20 min held at 95% B and 0.4 mL per min
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For example, you can use disinfectant products or bleach on common household surfaces