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In PTZs, substitutions at the C-10 position, such as alkyl or aryl groups, reduce activity, whereas modifications at the C-2 position significantly enhance potency, such as featuring a 2-phenyl-methyl piperazine at C-2, which demonstrated remarkable activity with an EC50 of 0.5 nM in erastin-induced ferroptosis in HT-1080 cells (Yang et al., 2021)

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