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After deeply anesthetized via intraperitoneal injection of Zoletil (40 mg/kg) and Xylazine (10 mg/kg), the animals were perfused transcardially with 0.9% saline followed by 300 mL of 4% paraformaldehyde in 0.1 M phosphate buffer (PB), pH 7.4

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The results of B12 Hydroxocobalamin 1mg/1ml injections can vary based on individual factors, such as metabolism and dietary habits